Past seminar
IAFP'S EUROPEAN SYMPOSIUM ON FOOD SAFETY 2023
Shaping the future of food safety since 2005, bringing together hundreds of food safety professionals from across Europe and around the workd to exchange ideas and gain knowledge about the latest in developments and techniques in food science and safety. The 2023 Symposium includes a vast array of diverse topics and speakers for those working in industry, government and academia.
Poster Session
Performance Evaluation of Ready-to-Use Culture Media, Easy Plate AC for Enumeration of Aerobic Plate Count in a Broad Range of Foods, Environmental Samples and Pet Foods
Abstract
Introduction: The Ready-to-use (RTU) media Easy Plate AC (Kikkoman Biochemifa Company) provides many benefits including reduced time-to- result, simplicity of us,e and is more sustainable compared to conventional plate count agar. The speed of analysis of the device gives a saving of 24 h over the reference method with results available in 48 h.
Purpose: To evaluate the performance of the RTU device for the enumeration of aerobic count as detailed in the ISO 16140-2 validation protocol.
Methods: The repeatability, accuracy and relative trueness of the RTU device was evaluated during the study across 5 food categories; dairy products, fishery products, produce and fruits, meat and poultry, and multicomponent foods in addition to environmental samples and pet foods. Analysis of the samples was performed according to the manufacturer’s instructions and ISO 4833-1 (2013).
Results: Study results revealed good agreement between the RTU device and the reference method in the 105 samples analyzed in the relative trueness study. During the accuracy profile it was demonstrated that all seven categories passed the 0.5 log acceptability limits or the recalculated limits. Additional studies showed that the RTU device prevented the spread of members of the Bacillus spp. seen on Plate Count Agar.
Significance: Data indicates that the RTU device provides equivalent results to the ISO reference method 4833-1 (2013) for a broad range of foods, environmental samples and pet foods. In addition to the reduced time-to-result compared to PCA, the media allows better colony counting with samples containing Bacillus spp.
Performance Evaluation of Ready-to-Use Culture Media, Easy Plate SA for Enumeration of Staphylococcus aureus in a Broad Range of Foods
Abstract
Introduction: Ready to use (RTU) media offers many advantages to the end user including shorter time-to-result and ease of use as well as being more sustainable. Easy Plate SA (Kikkoman Biochemifa Company) reduces the time of enumeration of Staphylococcus aureus to 24 h, a savings of 24 h compared to the standard ISO 6888-1 protocol.
Purpose: To compare the performance of the RTU device and ISO method 6888-1(2021) for the enumeration of S. aureus in a broad range of foods following the ISO 16140-2 validation protocol.
Methods: This study evaluated the specificity, selectivity, repeatability, accuracy and relative trueness of the RTU device as required by ISO 16140-2. During the study, 225 food samples were analyzed across five food categories: dairy products, fishery products, produce and fruits, meat and poultry and multicomponent foods. All samples were tested according to the manufacturer’s instructions and ISO 6888-1.
Results: Results from the study revealed the RTU device gave comparable results to the reference method across the 75 analyzed in the relative trueness with no evidence of bias between the methods. In the accuracy profile study, all five categories tested satisfied the 0.5 log acceptability limit or the recalculated acceptability limits. Data also indicated that the RTU device was selective and specific, inhibiting the growth of nine non-target organisms that grew on the reference agar.
Significance: The RTU device gives equivalent results to the ISO reference method 6888-1(2021) for a broad range of foods. This RTU device is a rapid convenient alternative for the enumeration of S. aureus enabling results in 24h.
ATP tests for hygiene monitoring are used in food processing because they are fast, easy to use, and cost-effective. Recent research has characterized the limitations of conventional ATP tests—limitations that can lead to false negative hygiene assessments. The issue relates to the tendency of ATP to degrade in many soils and under common plant environmental conditions. If this depletion occurs, ATP-based assessments become increasingly less sensitive and may yield indications that the surface in question is clean when, in fact, significant levels of soil may still remain. Conditions resulting in ATP depletion are most common in typical manufacturing venues and can result in a false negative in areas sensitive to food hygiene maintenance. This webinar will present recent research from the University of Wisconsin–Madison demonstrating this ATP-depletion phenomenon and how processors can guard against such false negative assessments.
Learning objectives:
- Why ATP tests may show false negative assessments
- Which food, and which food processors, are most at risk for these false negative assessments
- How this issue with ATP may be impacting your sanitation program
Result Contents:
Two leading experts discuss several detection methods, including ATP, ATP+AMP and A3, for efficient and rapid hygiene monitoring, and explore a new way to set benchmarks. Join Mikio Bakke and Dr Arne Lundin as they offer an insightful talk into hygiene control, designed for those in charge of hygiene monitoring systems, quality control/assurance, food safety, and/or sanitation programmes.
Attendees will learn:
- Find out about novel food and beverage hygiene tests, including Kikkoman A3, for the creation of an effective environmental monitoring programme (EMP)
- Discover the benefits of Kikkoman A3 as an ATP test
- Learn how to effectively set benchmarks for residues, including allergenic food.
More Than Just ATP: Monitoring Brewery Cleanliness with the Kikkoman A3 System
Ensuring that your brewery stays clean is more important than ever, and checking your brewing surfaces for contamination is the best way to keep production up and running. ATP technology is a fast and accurate method to check the cleanliness of any surface in your brewery. This webinar will outline the benefits of testing for ATP as part of your brewery sanitation program, and how the new A3 technology from Kikkoman goes beyond ATP testing alone. Join the team at Weber Scientific to learn more about using the A3 system to make sure your brewery stays clean!
This webinar was held on October 20, 2021 and is now available online!
Register below and watch the webinar for free.
