The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
| Origin | recombinant E. coli |
|---|---|
| Systematic name | Creatine amidinohydrolase |
| EC Number | 3.5.3.3 |
| Reaction formula | Creatine + H2O →→→ Sarcosine + Urea |
Creatinase (C2-AE)
- Enzymes for Clinical Chemistry
SPECIFICATION
| Appearance | white to light yellow lyophilizate | |
|---|---|---|
| Activity | ≧9 U/mg | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ |
PROPERTIES
| Molecular weight | ca. 80 kDa (gel filtration) |
|---|---|
| Structure | 2 subunits of 46 kDa (SDS-PAGE) |
| Michaelis constant | 1.3×10-2M (creatine) |
| pH Optimum | 7.0–9.0 |
| pH Stability | 5.0–11.0 |
| Optimum temperature | 40℃ |
| Thermal stability | below 45℃ |
| Stability (liquid form) | stable at 37℃ for at least two weeks |
| Stability (powder form) | stable at 30℃ for at least one month |
| Inhibitors | Hg2+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine and creatine in clinical analysis.
REFERENCES
Suzuki, M, Medical Technology, 7, 945–950 (1979).
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic determination of serum creatine
Clinica Chimica Acta, 140, 289–294.
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.