The enzyme is useful for the determination of cholesterol in clinical analysis.
| Origin | recombinant E. coli |
|---|---|
| Systematic name | Cholesterol : oxygen oxidoreductase |
| EC Number | 1.1.3 |
| Reaction formula | Cholesterol + 2O2 →→→ 6β-hydroperoxycholest-4-en-3-on + H2O2 |
| Appearance | yellow liquid | |
|---|---|---|
| Activity | ≧200 U/mL | |
| Contaminants | Catalase ≦2.0 U/U% | |
| pH | 7.3–7.7 | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ protected from light |
| Molecular weight | ca. 59 kDa (gel filtration) |
|---|---|
| Structure | monomer of 60 kDa (SDS-PAGE) |
| Michaelis constant | 1.9×10-5 M (cholesterol) |
| pH Optimum | 6.5–8.0 |
| pH Stability | 3.5–8.5 |
| Optimum temperature | 55℃–65℃ |
| Thermal stability | below 70℃ |
| Stability (liquid form) | stable at 25℃ for at least one month |
| Inhibitors | Ag+, Hg2+ |
The enzyme is useful for the determination of cholesterol in clinical analysis.
