Fructosyl-amino Acid Oxidase (FAOD-E)

  • Enzymes for Clinical Chemistry
Fructosyl-amino Acid Oxidase

The enzyme is useful for the determination of fructosyl-L-amino acid.

Origin recombinant E. coli
Systematic name

Fructosyl-L-amino acid : oxygen oxidoreductase
EC Number 1.5.3
Reaction formula

Fructosyl-L-amino acid + H2O + O2→→→ L-Amino acid + Glucosone + H2O2

SPECIFICATION

Appearance yellow lyophilizate
Activity ≧4.0 U/mg
Stabilizer trehalose
Storage condition below -20℃ protected from light

PROPERTIES

Molecular weight ca. 45 kDa (gel filtration)
Structure monomer of 50 kDa (SDS-PAGE)
Michaelis constant 2.2×10-4M (Nε-fructosyl-L-lysine)
pH Optimum 8.0–8.5
pH Stability 6.0–8.5
Optimum temperature 35–40℃
Thermal stability below 30℃
Stability (liquid form) stable at 25℃ for at least two weeks
Stability (powder form) stable at 37℃ for at least three weeks
Inhibitors Ag+，Cu2+
Specificity Nε-fructosyl-L-lysine (100), fructosyl-L-valine (65)
fructosyl-glycine (30)

APPLICATIONS

The enzyme is useful for the determination of fructosyl-L-amino acid.

REFERENCES

  • Horiuchi, T. et al., Agric. Biol. Chem., 53, 103–110 (1989).
  • Sakaue, R. et al., Biosci. Biotechnol. Biochem., 66, 1256–1261 (2002).
  • Hirokawa, K. and Kajiyama, N., Biosci. Biotechnol. Biochem., 66, 2323–2329 (2002).
  • Sakaue, R. and Kajiyama, N., Appl. Environ. Microbiol., 69, 139–145 (2003).



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