Fructosyl-amino Acid Oxidase (FAOD-E)
Frequently asked questions
- Where can I get Safety Data Sheet (SDS) of products?
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SDS and specification documents are available on each product page. They are also available from HERE. ⇒
Enzymes for Clinical Chemistry
- What precautions should be taken when handling the product after opening?
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To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)
- How should FAOD-E be stored?
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Please store at or below -20°C.
- How is the activity of FAOD-E measured?
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An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl valine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The prouction of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.
- What is the substrate specificity of FAOD-E?
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It shows 100% activity toward ε-fructosyl lysine (εF-Lys), 65% toward fructosyl valine (F-Val), and 30% toward fructosyl glycine (F-Gly).
- What stabilizers are used in FAOD-E?
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Trehalose is used as a stabilizer.
- How thermostable is FAOD-E?
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It is stable in liquid form below 30°C (Fig.4) and remains stable at 25°C for at least two weeks (Fig.5). In powder form, it remains stable at 37°C for at least three weeks (Fig.6).
- What are the optimal pH and temperature for FAOD-E?
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The optimal pH is 8.0–8.5, and the optimal temperature is 35–40°C.
- What is the molecular weight of FAOD-E?
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The molecular weight is approximately 45 kDa (gel filtration), and it exhibits a monomeric structure of 50 kDa (SDS-PAGE).
- What are the main applications of FAOD-E?
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It is useful for the determination of glycated albumin, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.