Fructosyl-amino Acid Oxidase (FAOD-E)

Frequently asked questions

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Enzymes for Clinical Chemistry

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FAOD-E be stored?

Please store at or below -20°C.

How is the activity of FAOD-E measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl valine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The prouction of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FAOD-E?

It shows 100% activity toward ε-fructosyl lysine (εF-Lys), 65% toward fructosyl valine (F-Val), and 30% toward fructosyl glycine (F-Gly).

What stabilizers are used in FAOD-E?

Trehalose is used as a stabilizer.

How thermostable is FAOD-E?

It is stable in liquid form below 30°C (Fig.4) and remains stable at 25°C for at least two weeks (Fig.5). In powder form, it remains stable at 37°C for at least three weeks (Fig.6).

What are the optimal pH and temperature for FAOD-E?

The optimal pH is 8.0–8.5, and the optimal temperature is 35–40°C.

What is the molecular weight of FAOD-E?

The molecular weight is approximately 45 kDa (gel filtration), and it exhibits a monomeric structure of 50 kDa (SDS-PAGE).

What are the main applications of FAOD-E?

It is useful for the determination of glycated albumin, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

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