Creatininase (C1-E)

  • Enzymes for Clinical Chemistry
Creatininase (C1-E)

The enzyme is useful for the determination of creatinine in clinical analysis.

Origin recombinant E. coli
Systematic name

Creatinine amidohydrolase

EC Number 3.5.2.10
Reaction formula

Creatinine H2O Creatine

SPECIFICATION

Appearance white lyophilizate
Activity 600-750 U/mg
Contaminants Catalase ≦0.5%
Stabilizer sucrose
Storage condition below -20℃

PROPERTIES

Molecular weight ca. 170 kDa (gel filtration)
Structure 6 subunits of 28 kDa (SDS-PAGE)
Isoelectric point 4.8
Michaelis constant 3.4×10-2M (creatinine)
4.3×10-2M ((creatine)
pH Optimum 6.5–7.0
pH Stability 7.0–11.0
Optimum temperature 60–65℃
Thermal stability below 60℃
Stability (liquid form) stable at 37°C for at least two weeks 
Stability (powder form) stable at 30°C for at least one month
Inhibitors Hg2+
Activators Mg2+,Mn2+ 

APPLICATIONS

The enzyme is useful for the determination of creatinine in clinical analysis.

REFERENCES

Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.



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