The enzyme is useful for the determination of creatinine in clinical analysis.
| Origin | recombinant E. coli |
|---|---|
| Systematic name | Creatinine amidohydrolase |
| EC Number | 3.5.2.10 |
| Reaction formula | Creatinine H2O Creatine |
Creatininase (C1-E)
- Enzymes for Clinical Chemistry
SPECIFICATION
| Appearance | white lyophilizate | |
|---|---|---|
| Activity | 600-750 U/mg | |
| Contaminants | Catalase ≦0.5％ | |
| Stabilizer | sucrose | |
| Storage condition | below -20℃ |
PROPERTIES
| Molecular weight | ca. 170 kDa (gel filtration) |
|---|---|
| Structure | 6 subunits of 28 kDa (SDS-PAGE) |
| Isoelectric point | 4.8 |
| Michaelis constant | 3.4×10-2M (creatinine) |
| 4.3×10-2M ((creatine) | |
| pH Optimum | 6.5–7.0 |
| pH Stability | 7.0–11.0 |
| Optimum temperature | 60–65℃ |
| Thermal stability | below 60℃ |
| Stability (liquid form) | stable at 37°C for at least two weeks |
| Stability (powder form) | stable at 30°C for at least one month |
| Inhibitors | Hg2+ |
| Activators | Mg2+，Mn2+ |
APPLICATIONS
The enzyme is useful for the determination of creatinine in clinical analysis.
REFERENCES
Suzuki, M. and Yoshida, M. (1984)
A new enzymatic serum creatinine measurement based on an endogenous creatine-eliminating system
Clinica Chimica Acta, 143, 147–155.