IAFP'S EUROPEAN SYMPOSIUM ON FOOD SAFETY 2024
Since 2005, IAFP's European Symposium has been shaping the future of food safety by providing a forum for the exchange of ideas with colleagues from across Europe working in industry, government, and academia. The Symposium is an excellent forum to gain knowledge about the latest developments and techniques in food science and safety. The symposium will be held 30 April - 2 May in Geneva, Switzerland.
Evaluation of Easy Plate EB Performance, a Novel Ready-to-Use Culture Medium for Detecting Enterobacteriaceae
Introduction :
Easy Plate is a Ready-to-Use (RTU) medium marketed as Medi-Ca since 2014 and rebranded by Kikkoman Biochemifa Company in 2021. Compared to conventional plate media, RTU medium offers various advantages, including faster time to results, ease of use, and sustainability.
Purpose:
In this study, the performance of a newly developed RTU medium for Enterobacteriaceae, Easy Plate EB (medium A), was evaluated by comparing it with three commercially available Enterobacteriaceae media in Japan: RTU media B and C and VRBG agar medium (VRBG).
Methods:
Both the inclusivity and exclusivity performance of 63 strains including 44 Enterobacteriaceae strains were assessed, and correlation analysis was conducted on 62 food matrices including meat, seafood, vegetables, dairy products, and prepared food. MALDI-TOF MS was used to identify bacterial species detected in samples that exhibited differences in colony count during correlation analysis.
Results:
All Enterobacteriaceae strains tested were positive, three of the non-Enterobacteriaceae strains tested were positive, and the other 16 tested negative. The correlation coefficients between medium A and VRBG, medium B, and medium C were 0.926, 0.988, and 0.963, respectively. The number of colonies that grew on medium A in 10 foods was 10 times higher than on VRBG, so the colonies were isolated, identified, and confirmed as Enterobacteriaceae. The isolated strains were pre-cultured in tryptic soy broth medium, they were tested in each medium and found to grow without significant differences in numbers.
Significance:
This new RTU medium for Enterobacteriaceae was found to have the same selective performance and high correlation with existing RTU media and agar medium. It was demonstrated that the method has the potential to detect bacteria in food more efficiently than VRBG.
Outline
| Product | Easy Plate EB |
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| Event date | 2024.04.30 |
Seminar Detail
| Venue | Geneva, Switzerland |
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Related Documents
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- Easy Plate EB Film medium for Enterobacteriaceae
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Easy Plate EB is a custom media formulation for cultivation of Enterobacteriaceae. A microbial test kit that contains Violet Red Bile Glucose (VRBG) media and a cold-water-soluble gelling agent that facilitates colony enumeration. The colonies are easily identified in 24±1 hours. Easy Plate EB is sold in packages of 100 tests in 4 separate bags containing 25 tests each. The shelf life is 12 months when stored unopened at 2-8℃.
Related seminars
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IAFP'S EUROPEAN SYMPOSIUM ON FOOD SAFETY 2023
Shaping the future of food safety since 2005, bringing together hundreds of food safety professionals from across Europe and around the workd to exchange ideas and gain knowledge about the latest in developments and techniques in food science and safety. The 2023 Symposium includes a vast array of diverse topics and speakers for those working in industry, government and academia.
Poster Session
Performance Evaluation of Ready-to-Use Culture Media, Easy Plate AC for Enumeration of Aerobic Plate Count in a Broad Range of Foods, Environmental Samples and Pet Foods
Abstract
Introduction: The Ready-to-use (RTU) media Easy Plate AC (Kikkoman Biochemifa Company) provides many benefits including reduced time-to- result, simplicity of us,e and is more sustainable compared to conventional plate count agar. The speed of analysis of the device gives a saving of 24 h over the reference method with results available in 48 h.Purpose: To evaluate the performance of the RTU device for the enumeration of aerobic count as detailed in the ISO 16140-2 validation protocol.
Methods: The repeatability, accuracy and relative trueness of the RTU device was evaluated during the study across 5 food categories; dairy products, fishery products, produce and fruits, meat and poultry, and multicomponent foods in addition to environmental samples and pet foods. Analysis of the samples was performed according to the manufacturer’s instructions and ISO 4833-1 (2013).
Results: Study results revealed good agreement between the RTU device and the reference method in the 105 samples analyzed in the relative trueness study. During the accuracy profile it was demonstrated that all seven categories passed the 0.5 log acceptability limits or the recalculated limits. Additional studies showed that the RTU device prevented the spread of members of the Bacillus spp. seen on Plate Count Agar.
Significance: Data indicates that the RTU device provides equivalent results to the ISO reference method 4833-1 (2013) for a broad range of foods, environmental samples and pet foods. In addition to the reduced time-to-result compared to PCA, the media allows better colony counting with samples containing Bacillus spp.
Performance Evaluation of Ready-to-Use Culture Media, Easy Plate SA for Enumeration of Staphylococcus aureus in a Broad Range of Foods
Abstract
Introduction: Ready to use (RTU) media offers many advantages to the end user including shorter time-to-result and ease of use as well as being more sustainable. Easy Plate SA (Kikkoman Biochemifa Company) reduces the time of enumeration of Staphylococcus aureus to 24 h, a savings of 24 h compared to the standard ISO 6888-1 protocol.Purpose: To compare the performance of the RTU device and ISO method 6888-1(2021) for the enumeration of S. aureus in a broad range of foods following the ISO 16140-2 validation protocol.
Methods: This study evaluated the specificity, selectivity, repeatability, accuracy and relative trueness of the RTU device as required by ISO 16140-2. During the study, 225 food samples were analyzed across five food categories: dairy products, fishery products, produce and fruits, meat and poultry and multicomponent foods. All samples were tested according to the manufacturer’s instructions and ISO 6888-1.
Results: Results from the study revealed the RTU device gave comparable results to the reference method across the 75 analyzed in the relative trueness with no evidence of bias between the methods. In the accuracy profile study, all five categories tested satisfied the 0.5 log acceptability limit or the recalculated acceptability limits. Data also indicated that the RTU device was selective and specific, inhibiting the growth of nine non-target organisms that grew on the reference agar.
Significance: The RTU device gives equivalent results to the ISO reference method 6888-1(2021) for a broad range of foods. This RTU device is a rapid convenient alternative for the enumeration of S. aureus enabling results in 24h.
