The enzyme is useful for the determination of urea in clinical analysis.
| Origin | jack bean |
|---|---|
| Systematic name | Urea amidohydrolase |
| EC Number | 3.5.1.5 |
| Reaction formula | Urea +H2O →→→ CO2 +2NH3 |
Urease (URE)
- Enzymes for Clinical Chemistry
SPECIFICATION
| Appearance | yellow lyophilizate | |
|---|---|---|
| Activity | ≧100 U/mg | |
| Stabilizer | lactose, ethylenediaminetetraacetic acid (EDTA), DTE | |
| Storage condition | below-20℃ |
PROPERTIES
| Molecular weight | ca. 480 kDa (gel filtration) |
|---|---|
| Structure | 8 subunits (containing nickel) |
| Isoelectric point | 5.0–5.1 |
| Michaelis constant | 1.05×10-2M (urea) |
| pH Optimum | ca. 8.0 |
| pH Stability | 5.0–10.0 |
| Optimum temperature | 60℃ |
| Thermal stability | below 50℃ |
| Inhibitors | heavy metal ions，Na+，K+，NH₄+，suramin, thiourea |
| Specificity | specific for urea |
APPLICATIONS
The enzyme is useful for the determination of urea in clinical analysis.
REFERENCES
- Sumner, J. B. and Hand, D. B., J. Am. Chem. Soc., 51, 1255–1260 (1929).
- Gorin, G. and Chin, C., Biochim. Biophys. Acta, 99, 418–426 (1965).
- Fishbein, W. N. et al., J. Biol. Chem., 245, 5985–5992 (1970).
- Fishbein, W. N. and Nagarajan, K., Arch. Biochem. Biophys., 144, 700–714 (1971).
- Contaxis, C. and Reithel, F. J., Canadian J. Biochem., 50, 461–473 (1972).
- Schlegel, H. and Kaltwasser, H., “Methods of Enzymatic Analysis,” Vol. 2 (2nd ed.), Verlag Chemie, Weinheim,Germany, 1974, pp. 1081–1085.
- Bergmeyer, H. U., “Methods of Enzymatic Analysis,” Vol. 2 (3rd ed.), Verlag Chemie, Weinheim, Germany, 1983,pp. 320–321.