Event Description 

This webinar will explore how the combination of Promedia, Easy Plate, and the Kikkoman Automatic Colony Counter System is revolutionizing microbiological testing. We will discuss the improvements in efficiency, accuracy, and automation offered by this integrated solution, from sample preparation to result interpretation, shaping the future of microbiology laboratories.

Easy Plate 

Save time, labor, and money with Kikkoman Easy Plate. Easy Plate is a convenient, prepared media plates for faster and easier microbiological testing. Using a time-tested and AOAC, MicroVal and NordVal validated culture media methodology, Easy Plate comes in an easy-to-use, stackable film plate format that saves on space, reduces waste, eliminates preparation time and labor, and streamlines your test procedures. Each Easy Plate is specialized for a specific target organism (or group) and includes options for Aerobic Bacteria (AC), Coliforms (CC), E. coli/Coliforms (EC), Staphylococcus aureus (SA), Yeast and Mold(YM-R) and Enterobacteriaceae(EB) and Rapid Type for Aerobic Bacteria (AC-R).

Colony Counting System 

The new solution by Kikkoman Biochemifa Company for colony counting is available. The software “Colony Counting System for Easy Plate” can be downloaded for free. Automatically counts and analyzes the colonies in the imaged Easy Plate. Simpler and time-saving way to count colonies than manual counting advances efficiency and eliminates the effects of variation and skill level among operators.

Speaker 

Mr. Anjas Wilapangga: Senior Analyst PT Kinglab Indonesia 

IAFP European Symposium 2025 – Technical Session 9

T9: Evaluation of Easy Plate YM-R™ for Enumerating Yeasts and Molds in Both High and Low Water Activity Foods

Introduction

Kikkoman Biochemifa Company will be presenting new validation data for Easy Plate YM-R™, a ready-to-use (RTU) culture medium developed for the rapid enumeration of yeasts and molds. The product enables results in 48 to 72 hours and is suitable for both high and low water activity foods. This presentation will take place as part of Technical Session 9 (T9) at the IAFP European Symposium 2025.

Purpose

The purpose of this study is to evaluate the performance of Easy Plate YM-R™ in alignment with AOAC Performance Tested Methods℠, MicroVal, and NordVal certification requirements, with particular focus on applied statistical methods for method validation.

Study Overview

Methodology:

Easy Plate YM-R™ was compared against ISO 21527-1/2 (2008) and BAM Chapter 18 (2001). Validation was conducted across diverse product categories including:

• Dairy
• Fruits and produce
• Multicomponent foods
• Bakery items
• Meat and poultry
• Dried foods
• Confectionery
• Pet foods
• Environmental swabs

Parameters assessed include:

• Relative trueness: 135 items using Bland-Altman plots
• Repeatability and accuracy: β-ETIs and confidence intervals (19 items)
• Selectivity: 50 inclusivity strains, 30 exclusivity strains Robustness, consistency, and stability: via ANOVA

Key Findings

• At 48 hours, Easy Plate YM-R™ demonstrated agreement with reference methods in three categories: produce, multicomponent foods, and environmental samples
• At 72 hours, consistent agreement was achieved across all tested food types, within ±0.5 log or recalculated acceptance limits
• Selectivity surpassed reference methods, with the medium supporting growth of all tested xerophilic and non-xerophilic inclusivity strains, and inhibiting all exclusivity strains
• Robustness testing indicated the importance of maintaining correct incubation temperature
• Consistency and long-term product stability were also confirmed

Conclusion

Easy Plate YM-R™ is a robust and time-saving alternative to traditional yeast and mold testing methods. It offers a simplified workflow, wide applicability across food matrices, and strong statistical validation. The method is especially valuable for manufacturers managing both high and low water activity products.

Presenters

• Shinichiro Sugiura – Primary Author and Presenter, Kikkoman Biochemifa Company
• Daiki Utsunomiya – Kikkoman Biochemifa Company
• Alice Foxall – Campden BRI
• Suzanne Jordan – Campden BRI

We welcome IAFP EU 2025 attendees to join Technical Session 9 (T9) to learn more about the validation and application of Easy Plate YM-R™ in microbiological testing. For additional information or to request a meeting during the event, please contact us through our [inquiry form] or speak with your local representative.

IAFP European Symposium 2025 – Poster Session P1-69

Evaluation of Accuracy and Efficiency for Novel Automatic Colony Counting System for Ready-to-Use Culture Media, Easy Plate™

Introduction

Ready-to-use (RTU) culture media improves food safety testing by offering quicker preparation, compact form, easy handling, and clearer visibility of colonies compared to traditional agar media. However, manual colony counting remains labor-intensive and requires trained personnel. To enhance efficiency, an automated colony counting solution is necessary.

Purpose

This study aimed to evaluate the rapidity and accuracy of a new Colony Counting System (CCS) developed for Easy Plate™ (Kikkoman Biochemifa Company). The CCS uses a general document scanner with an automatic feeder and dedicated analysis software based on object detection through machine learning.

Method

Ten types of food samples were diluted and plated on Easy Plate media for Aerobic Count (AC) and Coliform Count (CC) testing. Three levels of contamination were prepared, and results from the CCS were compared with conventional manual counting in terms of processing time and colony count accuracy.

Results

The CCS processed each sheet in approximately 5.0 to 6.4 seconds, regardless of bacterial load. In contrast, manual counting took between 13.8 and 44.5 seconds per sheet, especially longer at lower contamination levels (10–50 CFU/sheet). The correlation coefficient between the CCS and manual counting was over 0.98 for both AC and CC tests. These findings indicate that the CCS can reduce counting time by approximately 77% without compromising accuracy.

Significance

Combining ready-to-use culture media with an automated counting system provides an efficient, accurate, and cost-saving approach to microbiological testing. This innovation is particularly valuable for high-throughput environments in food safety laboratories.

Presenters

• Shinichiro Sugiura, Primary Author, Kikkoman Biochemifa Company
• Kentaro Takenaka, Abstract Presenter, Kikkoman Corporation