FAQ (Category)

Frequently asked questions

How long is the expiration date of LuciPac?

Before opening, it is stable at 2-8 ° C for 15 months after manufacture. The shelf life is printed on the bottom of the LuciPac aluminum bag. After opening the aluminum bag, store it in a refrigerator and use it within 2 weeks. Please also see the precautions for use.

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Where can I buy Test kit?

We sell through distributors. Please check here for the distributors of ATP Rapid Hygiene Monitoring products.

Is it possible for the measured value of the Lumitester to be "0"?

The tap water that wets the cotton balls also has ATP, ADP, and AMP, so the measured value never reaches "0". If the measured value becomes "0", there may be detergent, bleach, etc. that interfere with the measurement at the inspection site. It is also possible that there was a problem with the operation of the Lumitester.

Enzymes for Clinical Chemistry

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should βPGM-EP be stored?

Please store at or below -20°C.

What stabilizers are used in βPGM-EP?

Lactose and EDTA are used as stabilizers.

How thermostable is βPGM-EP?

It is stable in liquid form below 45°C (Fig.4) and remains stable at 37°C for at least one week (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for βPGM-EP?

The optimal pH is approximately 7.0, and the optimal temperature is 40°C.

What is the molecular weight of βPGM-EP?

The molecular weight is approximately 34 kDa (gel filtration), and it exhibits a monomeric structure of 25 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should U-TE be stored?

Please store at or below -20°C.

What stabilizers are used in U-TE?

Sucrose, citrate, and EDTA are used as stabilizers.

How thermostable is U-TE?

It is stable in liquid form below 60°C (Fig.4) and remains stable at 37°C for at least ten days (Fig.5). In powder form, it remains stable at 30°C for at least three weeks (Fig.6).

What are the optimal pH and temperature for U-TE?

The optimal pH is 7.0–8.0, and the optimal temperature is 37–45°C.

What is the molecular weight of U-TE?

The molecular weight is approximately 90 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 35 kDa each.

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should SPL-E be stored?

Please store at or below -20°C.

What stabilizers are used in SPL-E?

Sucrose is used as a stabilizer.

How thermostable is SPL-E?

It is stable in liquid form below 45°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least two weeks (Fig.6).

What are the optimal pH and temperature for SPL-E?

The optimal pH is 7.5, and the optimal temperature is 40°C.

What is the molecular weight of SPL-E?

It exhibits a monomeric structure with a molecular weight of approximately 56 kDa (gel filtration and SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should SOD-TE be stored?

Please store at or below -20°C.

What stabilizers are used in SOD-TE?

Sucrose is used as a stabilizer.

How thermostable is SOD-TE?

It is stable in liquid form below 55°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for SOD-TE?

The optimal pH is 6.7–9.5, and the optimal temperature is 50°C.

What is the molecular weight of SOD-TE?

The molecular weight is approximately 49 kDa (gel filtration), and it exhibits a monomeric structure of 43 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should SOD-EP be stored?

Please store at or below -20°C.

What stabilizers are used in SOD-EP?

Sucrose is used as a stabilizer.

How thermostable is SOD-EP?

It is stable in liquid form below 55°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for SOD-EP?

The optimal pH is 7.5, and the optimal temperature is 50°C.

What is the molecular weight of SOD-EP?

The molecular weight is approximately 49 kDa (gel filtration), and it exhibits a monomeric structure of 43 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should MPL-EP be stored?

Please store at or below -20°C.

What stabilizers are used in MPL-EP?

Lactose and EDTA are used as stabilizers.

How thermostable is MPL-EP?

It is stable in liquid form below 55°C (Fig.4) and remains stable at 37°C for at least one week (Fig.5). In powder form, it remains stable at 30°C for at least four weeks (Fig.6).

What are the optimal pH and temperature for MPL-EP?

The optimal pH is 6.5–7.5, and the optimal temperature is 45–50°C.

What is the molecular weight of MPL-EP?

The molecular weight is approximately 220 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 90 kDa each.

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should LDH-E be stored?

Please store at or below -20°C.

How thermostable is LDH-E?

It is stable below 40°C in liquid form (Fig.4).

What are the optimal pH and temperature for LDH-E?

The optimal pH is 7.5, and the optimal temperature is 40°C.

What is the molecular weight of LDH-E?

It exhibits a tetrameric structure composed of subunits of 55 kDa each (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should GST be stored?

Please store at or below -20°C.

What is the isoelectric point of GST?

The isoelectric point is pH 6.5.

What stabilizers are used in GST?

Sucrose is used as a stabilizer.

How thermostable is GST?

It is stable in liquid form below 40°C (Fig.4).

What are the optimal pH and temperature for GST?

The optimal pH is 7.0, and the optimal temperature is 60°C.

What is the molecular weight of GST?

The molecular weight is approximately 900 kDa (gel filtration), and it exhibits a multimeric structure composed of subunits of 57 kDa each (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should GLOD-E be stored?

Please store at or below -20°C.

What stabilizers are used in GLOD-E?

Raffinose is used as a stabilizer.

How thermostable is GLOD-E?

It is stable in liquid form below 55°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for GLOD-E?

The optimal pH is 6.0–8.0, and the optimal temperature is 40–50°C.

What is the molecular weight of GLOD-E?

The molecular weight is approximately 115 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 70 kDa each.

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should GLN be stored?

Please store at or below -20°C.

What is the substrate specificity of GLN?

It shows 100% activity toward L-glutamine, 70% toward D-glutamine, and 0% toward L-asparagine and D-asparagine.

What stabilizers are used in GLN?

Sucrose is used as a stabilizer.

How thermostable is GLN?

It is stable in liquid form below 50°C (Fig.4) and remains stable at 37°C for at least four days (Fig.5). In powder form, it remains stable at 30°C for at least two weeks (Fig.6).

What are the optimal pH and temperature for GLN?

The optimal pH is 7.0, and the optimal temperature is 65°C.

What is the molecular weight of GLN?

The molecular weight is approximately 58 kDa (gel filtration), and it exhibits a heterodimeric structure composed of 45 kDa and 22 kDa subunits (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should GLDH-R be stored?

Please store at or below -20°C.

What stabilizers are used in GLDH-R?

Trehalose is used as a stabilizer.

How thermostable is GLDH-R?

It is stable in liquid form below 55°C (Fig.4). In powder form, it remains stable at 37°C for at least two weeks.

What are the optimal pH and temperature for GLDH-R?

The optimal pH is 8.5–9.0, and the optimal temperature is 35–45°C.

What is the molecular weight of GLDH-R?

It exhibits a monomeric structure with a molecular weight of approximately 49 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FPOX-CET be stored?

Please store at or below -20°C.

How is the activity of FPOX-CET measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl glycine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The production of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FPOX-CET?

It shows 100% activity toward fructosyl valyl histidine (F-ValHis), 150% toward Fructosyl-glycine (F-Gly) and 68.6% toward ε-fructosyl lysine (εF-Lys).

What protease is used in combination with FPOX-CET for HbA1c measurement?

Please contact sales representative.

What stabilizers are used in FPOX-CET?

Sodium glutamate and EDTA are used as stabilizers.

How thermostable is FPOX-CET?

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at -20°C for at least twelve months (Fig.5).

What are the optimal pH and temperature for FPOX-CET?

The optimal pH is 7.5–8.5, and the optimal temperature is 37–45°C.

What is the molecular weight of FPOX-CET?

The molecular weight is approximately 60 kDa (gel filtration), and it exhibits a monomeric structure of 52 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FPOX-CE be stored?

Please store at or below -20°C.

How is the activity of FPOX-CE measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl glycine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The production of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FPOX-CE?

It shows 100% activity toward fructosyl valyl histidine (F-ValHis), 84% toward ε-fructosyl lysine (εF-Lys) and 53% toward Fructosyl-glycine (F-Gly).

What protease is used in combination with FPOX-CE for HbA1c measurement?

Please contact sales representative.

What stabilizers are used in FPOX-CE?

Sodium glutamate and EDTA are used as stabilizers.

How thermostable is FPOX-CE?

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at 37°C for at least one month (Fig.5).

What are the optimal pH and temperature for FPOX-CE?

The optimal pH is 7.5–8.0, and the optimal temperature is 35–42°C.

What is the molecular weight of FPOX-CE?

The molecular weight is approximately 60 kDa (gel filtration), and it exhibits a monomeric structure of 52 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FAOD-E be stored?

Please store at or below -20°C.

How is the activity of FAOD-E measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl valine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The prouction of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FAOD-E?

It shows 100% activity toward ε-fructosyl lysine (εF-Lys), 65% toward fructosyl valine (F-Val), and 30% toward fructosyl glycine (F-Gly).

What stabilizers are used in FAOD-E?

Trehalose is used as a stabilizer.

How thermostable is FAOD-E?

It is stable in liquid form below 30°C (Fig.4) and remains stable at 25°C for at least two weeks (Fig.5). In powder form, it remains stable at 37°C for at least three weeks (Fig.6).

What are the optimal pH and temperature for FAOD-E?

The optimal pH is 8.0–8.5, and the optimal temperature is 35–40°C.

What is the molecular weight of FAOD-E?

The molecular weight is approximately 45 kDa (gel filtration), and it exhibits a monomeric structure of 50 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FADGDH-AD be stored?

Please store at or below -20°C.

How is the activity of FADGDH-AD measured?

An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.

What is the substrate specificity of FADGDH-AD?

It shows less than 1% relative activity toward maltose, xylose, and galactose compared to 100% for glucose.

What stabilizers are used in FADGDH-AD?

No stabilizers are used.

How thermostable is FADGDH-AD?

It is stable in liquid form below 60°C (Fig.4). In powder form, it remains stable at 30°C for at least one month (Fig.5).

What are the optimal pH and temperature for FADGDH-AD?

The optimal pH is 7.0–7.5, and the optimal temperature is 45°C.

What is the molecular weight of FADGDH-AD?

It exhibits a monomeric structure with a molecular weight of approximately 90 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FADGDH-AB be stored?

Please store at or below -20°C.

How is the activity of FADGDH-AB measured?

An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.

What is the substrate specificity of FADGDH-AB?

It shows less than 1% relative activity toward maltose compared to 100% for glucose.

What stabilizers are used in FADGDH-AB?

No stabilizers are used.

How thermostable is FADGDH-AB?

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at 30°C for at least 20 days (Fig.5).

What are the optimal pH and temperature for FADGDH-AB?

The optimal pH is 7.0–7.5, and the optimal temperature is 40–50°C.

What is the molecular weight of FADGDH-AB?

It exhibits a monomeric structure with a molecular weight of approximately 85 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FADGDH-AA be stored?

Please store at or below -20°C.

How is the activity of FADGDH-AA measured?

An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.

What is the substrate specificity of FADGDH-AA?

It shows less than 1% relative activity toward maltose, xylose, and galactose compared to 100% for glucose.

What stabilizers are used in FADGDH-AA?

No stabilizers are used.

How thermostable is FADGDH-AA?

It is stable in liquid form below 50°C (Fig.4).

What are the optimal pH and temperature for FADGDH-AA?

The optimal pH is 7.0–7.5, and the optimal temperature is 40–50°C.

What is the molecular weight of FADGDH-AA?

It exhibits a monomeric structure with a molecular weight of approximately 90 kDa (SDS-PAGE).

How should CHO-PEWL be stored?

Please store at or below -20°C.

What stabilizers are used in CHO-PEWL?

Sucrose is used as a stabilizer.

How thermostable is CHO-PEWL?

It is stable in liquid form below 70°C (Fig.4) and remains stable at 25°C for at least one month (Fig.5).

What are the optimal pH and temperature for CHO-PEWL?

The optimal pH is 6.5–8.0, and the optimal temperature is 55–65°C.

What is the molecular weight of CHO-PEWL?

The molecular weight is approximately 59 kDa (gel filtration), and it exhibits a monomeric structure of 60 kDa (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should CHO-CE be stored?

Please store at or below -20°C.

What stabilizers are used in CHO-CE?

Trehalose, sodium malate, sodium glutamate, and EDTA are used as stabilizers.

How thermostable is CHO-CE?

It is stable in liquid form below 55°C (Fig.4) and remains stable at 37°C for at least one week (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for CHO-CE?

The optimal pH is 7.0, and the optimal temperature is 50°C.

What is the molecular weight of CHO-CE?

The molecular weight is approximately 58 kDa (gel filtration), and it exhibits a monomeric structure (55 kDa by SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should C2-EP be stored?

Please store at or below -20°C.

What stabilizers are used in C2-EP?

Sucrose is used as a stabilizer.

How thermostable is C2-EP?

It is stable in liquid form below 50°C (Fig.4) and remains stable at 37°C for at least three weeks (Fig.5). In powder form, it remains stable at 30°C

What are the optimal pH and temperature for C2-EP?

The optimal pH is 7.0–9.0, and the optimal temperature is 45°C.

What is the molecular weight of C2-EP?

The molecular weight is approximately 80 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 48 kDa each (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should C2-AT be stored?

Please store at or below -20°C.

What stabilizers are used in C2-AT?

Sucrose is used as a stabilizer.

How thermostable is C2-AT?

It is stable in liquid form below 53°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for C2-AT?

The optimal pH is 7.0–9.0, and the optimal temperature is 45°C.

What is the molecular weight of C2-AT?

The molecular weight is approximately 80 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 48 kDa each (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should C2-AE be stored?

Please store at or below -20°C.

What stabilizers are used in C2-AE?

Sucrose is used as a stabilizer.

How thermostable is C2-AE?

It is stable in liquid form below 45°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for C2-AE?

The optimal pH is 7.0–9.0, and the optimal temperature is 40°C.

What is the molecular weight of C2-AE?

The molecular weight is approximately 80 kDa (gel filtration), and it exhibits a dimeric structure composed of subunits of 46 kDa each (SDS-PAGE).

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should C1-E be stored?

Please store at or below -20°C.

What stabilizers are used in C1-E?

Sucrose is used as a stabilizer.

How thermostable is C1-E?

It is stable in liquid form below 60°C (Fig.4) and remains stable at 37°C for at least two weeks (Fig.5). In powder form, it remains stable at 30°C for at least one month (Fig.6).

What are the optimal pH and temperature for C1-E?

The optimal pH is 6.5–7.0, and the optimal temperature is 60–65°C.

What is the molecular weight of C1-E?

The molecular weight is approximately 170 kDa (gel filtration), and it exhibits a hexameric structure composed of subunits of 28 kDa each (SDS-PAGE).

How should ALP-A be stored?

Please store at or below -20°C.

How thermostable is ALP-A?

It is stable in liquid form below 50°C (Fig.4) and remains stable at 37°C for at least one week (Fig.5).

What are the optimal pH and temperature for ALP-A?

The optimal pH is 9.5–10, and the optimal temperature is 50°C.

What is the molecular weight of ALP-A?

The molecular weight is approximately 106 kDa (gel filtration).

What are the main applications of βPGM-EP?

It is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

What are the main applications of U-TE?

It is useful for the determination of uric acid in clinical analysis.

What are the main applications of SPL-E?

It is useful for the determination of inorganic phosphate in clinical analysis.

What are the main applications of SOD-TE?

It is useful for the determination of creatinine and creatine in clinical analysis.

What are the main applications of SOD-EP?

It is useful for the determination of creatinine and creatine in clinical analysis.

What are the main applications of MPL-EP?

It is useful for the determination of α-amylase and inorganic phosphate in clinical analysis.

What are the main applications of LDH-E?

It is useful for the determination of lactate in clinical analysis and continuous lactate sensors.

What are the main applications of GST?

It is useful for the determination and removal of ammonia in clinical analysis.

What are the main applications of GLOD-E?

It is useful for the determination of L-glutamate.

What are the main applications of GLN?

It is useful for the determination of glutamine in clinical analysis.

What are the main applications of GLDH-R?

It is useful for BUN (blood urea nitrogen) testing and elimination of free ammonia.

What are the main applications of FPOX-CET?

It is useful for the determination of HbA1c, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

What are the main applications of FPOX-CE?

It is useful for the determination of HbA1c, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

What are the main applications of FAOD-E?

It is useful for the determination of glycated albumin, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

What are the main applications of FADGDH-AD?

It is useful for the determination of blood glucose levels in diabetes diagnosis, particularly for continuous glucose monitoring, with minimal interference from substances such as maltose and xylose.

What are the main applications of FADGDH-AB?

It is useful for the determination of blood glucose levels in diabetes diagnosis, with minimal interference from substances such as maltose.

What are the main applications of FADGDH-AA?

It is useful for the determination of blood glucose levels in diabetes diagnosis, with minimal interference from substances such as maltose and xylose.

What are the main applications of CHO-PEWL?

It is useful for the determination of cholesterol in clinical analysis.

What are the main applications of CHO-CE?

It is useful for the determination of cholesterol in clinical analysis.

What are the main applications of C2-EP?

It is useful for the determination of creatinine and creatine in clinical analysis.

What are the main applications of C2-AT?

It is useful for the determination of creatinine and creatine in clinical analysis.

What are the main applications of C2-AE?

It is useful for the determination of creatinine and creatine in clinical analysis.

What are the main applications of C1-E?

It is useful for the determination of creatinine in clinical analysis.

What are the main applications of ALP-A?

It is widely used as a marker in ALP-based immunoassays.

ATP Test

What are Relative Light Units (RLU)? 

The principle of the ATP test is based on the firefly luciferase reaction, which can produce light using luciferin and ATP. The amount of light produced is proportional to the amount of ATP in a sample and can be quantified by measuring the light. The results are displayed in relative light units (RLU). RLU are not a standardized unit of measurement like inches or centimeters, and each manufacturer sets their own value for 1 RLU based on their reagent formulations and light detection systems. Therefore, a system that can indicate larger RLU values for a certain amount of ATP is not necessarily more sensitive. Benchmark RLU values are set to determine regular cleanliness in a facility. They are generally set from tens to thousands of RLU depending on sites.

How should I dispose of LuciPac?

No sterilization required. Dispose of according to local plastic disposal regulations. The main body is made of polypropylene, and glass and PVC (polyvinyl chloride) are not used. (For details, see the Lucipac Instruction Manual PDF [Disposal Method])"

Is it possible for the measured value to be "0"?

The tap water that wets the cotton balls also has ATP, ADP, and AMP, so the measured value never reaches "0". If the measured value becomes "0", there may be detergent, bleach, etc. that interfere with the measurement at the inspection site. It is also possible that there was a problem with the operation of the Lumitester.

How long is the expiration date of LuciPac?

Before opening, it is stable at 2-8℃ for 15 months after manufacturing date. The shelf life is printed on the bottom of the LuciPac aluminum bag.

After opening the aluminum bag, store it in a refrigerator and use it within 2 weeks.

Please also see the precautions for use.https://biochemifa.kikkoman.com/e/kit/method/atp-test-20/

How do I set the standard value for ATP Test?

Standard (benchmark) values are RLU levels that are used to determine the pass-fail criteria for cleanliness levels controls for the test being conducted. Standard values are set using either specific site data or recommendations. Based on the data collected during sampling, Standard Values 1 and 2 are set to develop Action Levels (Figure 1) with tests below Value 1 being a “Pass” test, indicating that cleaning processes are being effective according to the validated methods, above Value 1 but below Value 2 serving as a “Caution” range, indicating that adherence to cleaning processes may be deteriorating and the cause should be investigated, and finally, levels above Value 2 indicate that cleaning has not been effective and recleaning is appropriate.
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The ATP Test (Kikkoman A3)

How should I dispose of LuciPac?

No sterilization required. Dispose of according to local plastic disposal regulations. The main body is made of polypropylene, and glass and PVC (polyvinyl chloride) are not used. (For details, see the Lucipac Instruction Manual PDF [Disposal Method])"

Is it possible for the measured value to be "0"?

The tap water that wets the cotton balls also has ATP, ADP, and AMP, so the measured value never reaches "0". If the measured value becomes "0", there may be detergent, bleach, etc. that interfere with the measurement at the inspection site. It is also possible that there was a problem with the operation of the Lumitester.

How long is the expiration date of LuciPac?

Before opening, it is stable at 2-8℃ for 15 months after manufacturing date. The shelf life is printed on the bottom of the LuciPac aluminum bag.

After opening the aluminum bag, store it in a refrigerator and use it within 2 weeks.

Please also see the precautions for use.https://biochemifa.kikkoman.com/e/kit/method/atp-test-20/

Does the Lumitester Smart need to be adjusted to zero point before each measurement?

No need to adjust the zero point for each measurement. AUTO ZERO calibration function is built-in for Lumitester Smart.

If the calibration for Lumitester is required by ISO or other regulations please contact your supplier you have purchased from. 

What can be the reason for the extremely low RLU (e.g., 0 RLU) results compared to usual. What is affecting the results?

There are 3 possible reasons that affects the measurements.

1) The test point or test sample contain alcohol, detergent, bleach, or other inhibitors, it may result lower RLU than usual. 

In some cases, it may show 0 RLU value if high concentrated detergent left on the test surface.

2) LuciPac was measured immediately after it was taken out of the refrigerator, it may also lower the RLU value.

3) Shutter malfunction in the Lumitester Smart. When there is a shutter malfunction inside the chamber, there is a possibility that it will effect the measurements.  

Why is ATP Test (Kikkoman A3) a effective hygiene monitoring system at food / beverage factory and health care center?

In food / beverage factory and health care center, it is important to quickly check the sanitary conditions.

The ATP Test (Kikkoman A3) measures the amount of ATP+ADP+AMP contained in soil/ residues and bacteria.

The cleanliness is instantly displayed as a numerical value of the tested area, on-site. 

What is the uniquness of ATP Test (Kikkoman A3) compared to the other ATP test?

ATP Test (Kikkoman A3) uses the unique enzymatic reactions in the proprietary A3 chemistry of our LuciPac reagents to change ADP and AMP to ATP enabling

the measurement of the total amount of ATP + ADP + AMP, producing a significantly more highly sensitive measurement for the detection of residual soil which can be missed when measuring ATP only.

Does detergent, bleach, sanitizer, disinfectant effect the RLU results?

Yes, it may. Any detergent, bleach (sodium hypochlorite), alcohol or other disinfectant, or disinfectant left on the surface may affect the results.

Degree of effect will depend on the inhibitory substances. If the inhibitor is rinsed thoroughly, there will be no effect in most cases.

For alcohol, there will be no effect when they are fully evaporated.

For more information: https://biochemifa.kikkoman.com/e/kit/method/atp-test-20/

*ATP Test (Kikkoman A3) is recommended to be performed after cleaning and before disinfection.

Can RLU counts be converted/calculaterd into CFU?

RLU reults cannot be replaced by the number of bacteria. The ATP Test (Kikkoman A3) measures the

ATP+ADP+AMP derived from soil/ residue and bacteria at the same time.

Even if the bacteria is present, the amount of ATP+ADP+AMP each bacteria has differs depending on the type and condition of the bacteria.

It is impossible to determine how many bacteria it corresponds. It may show corelation only when pippeting microorganisms and perform measurement in dilution series.

Is there correlation between the RLU and CFU?

There are no correlation between RLU and CFU plate counts for the typical condition/ surface.

There are microorganisms and ATP+ADP+AMP derived from soil/ residue when swabbing environmental surfaces. 

For more information: https://biochemifa.kikkoman.com/e/kit/method/atp-test-03/

Is there any soil / residue that ATP Test (Kikkoman A3) cannot measure?

Kikkoman A3 detects the amount of ATP+ADP+AMP. Any soil/ residue that doesn't include ATP, ADP and AMP would not be measured. 

*Viruses, which are not technically living cells, usually do not contain ATP

Kikkoman Biochemifa is closely monitoring an outbreak of coronavirus ( COVID-19 ).

*Apart from viruses, microorganisms and other organic substances contain ATP. Consequently, it is effective for daily evaluation of cleaning

Why does ATP need to be measured?

How often should I calibrate my Lumitester?

We do not have a recommended calibration frequency for Lumitester. 

Calibration is important because of audits, and internal standard operating procedures. We will recommened to follow your company's procedure and recommendations. We  offer a Lumitester Calibration Kit for a self-inspection, that allows you to easily check the operation and sensitivity of the Lumitester. If manufacturer's calibration is required as a part of your quality program please contact us for further information. 

Are there repair and callibration services for Lumitester?

Repair and calibration services are available as follows:

(1) Repair : Lumitester Smart, PD-30, Lumitester Calibration Kit 

(2) Calibration: Lumitester Smart, PD-30, Lumitester Calibration kit

(3) COA : Lumitester Smart, Lumitester Calibration kit

Please contact your supplier for quotation.

What are the advantages of the ATP Test (Kikkoman A3)?

Since organic debris onsite contains remarkably large amount of ADP and AMP, the A3 Test can detect organic debris more effectively than conventional ATP tests. Accordingly, one can see that the primary consideration for selection of an ATP tests to manage the effectiveness of cleaning is the ability of the test to detect ADP and AMP as well as ATP rather than the precision of the test’s detection of pure ATP.
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