CheckLite 250 Plus

For ATP measurement in microorganisms


  • CheckLite 250 Plus is a kit for microbial biomass assays based on the ATP measuring method.
  • CheckLite contains thermostable firefly luciferase.
  • CheckLite is characterized by its ease of use and rapid performance.
  • CheckLite can detect 10,000 cells of coliforms/ml (1,000 cell/assay).

Composition of kit

Product Name CheckLite 250 Plus
Product Code 61305
Number of measurements 250 assays

Luciferin-luciferase reagent (lyophilized) x 5

Reconstitution buffer (5.5 ml) x 5

ATP releasing reagent (5.5 ml) x 5

Storage 2-8 ℃
Expiry End of month after 16 months from manufacturing date.


  • CheckLite 250 Plus should be stored at 2-8 ℃ in a refrigerator. DO NOT FREEZE.


  1. A ratio of spore to viable organisms: A case study of the JPL – SAF cleanroom. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA. 2017.
  2. Development of a filtration-based bioluminescence assay for detection of microorganisms in tea beverages. J. Food Prot. 2016, 79, 488-492.
  3. Rapid detection of bacteria in green tea using a novel pretreatment method in a bioluminescence assay. J. Food Prot. 2014, 77, 941-947.
  4. Evaluation of an improved bioluminescence assay for the detection of bacteria in soy milk. Biocontrol Sci. 2013, 18, 1-7.
  5. Sensitive detection of bacteria and spores using a portable bioluminescence ATP measurement assay system distinguishing from white powder materials. J. Health Sci. 2004, 50, 126-132.
  6. ATP as a biomarker of viable microorganisms in clean-room facilities. J. Microbiol. Methods 2003, 52, 367-377.