Glucose Dehydrogenase (FADGDH-AD)
Frequently asked questions
- How can I have "SDS”(Safety Data Sheet for Chemical Substances) and "Specifications"?
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Please fulfill the request form in the "SDS request form" page.
You can find the page, either from the products information site or just click the link below for direct linkage.
https://biochemifa.kikkoman.co.jp/e/support/sds/
Enzymes for Clinical Chemistry
- What precautions should be taken when handling the product after opening?
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To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)
- How should FADGDH-AD be stored?
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Please store at or below -20°C.
- How is the activity of FADGDH-AD measured?
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An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.
- What is the substrate specificity of FADGDH-AD?
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It shows less than 1% relative activity toward maltose, xylose, and galactose compared to 100% for glucose.
- What stabilizers are used in FADGDH-AD?
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No stabilizers are used.
- How thermostable is FADGDH-AD?
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It is stable in liquid form below 60°C (Fig.4). In powder form, it remains stable at 30°C for at least one month (Fig.5).
- What are the optimal pH and temperature for FADGDH-AD?
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The optimal pH is 7.0–7.5, and the optimal temperature is 45°C.
- What is the molecular weight of FADGDH-AD?
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It exhibits a monomeric structure with a molecular weight of approximately 90 kDa (SDS-PAGE).
- What are the main applications of FADGDH-AD?
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It is useful for the determination of blood glucose levels in diabetes diagnosis, particularly for continuous glucose monitoring, with minimal interference from substances such as maltose and xylose.