Glucose Dehydrogenase (FADGDH-AA)

Frequently asked questions

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

Enzymes for Clinical Chemistry

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FADGDH-AA be stored?

Please store at or below -20°C.

How is the activity of FADGDH-AA measured?

An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.

What is the substrate specificity of FADGDH-AA?

It shows less than 1% relative activity toward maltose, xylose, and galactose compared to 100% for glucose.

What stabilizers are used in FADGDH-AA?

No stabilizers are used.

How thermostable is FADGDH-AA?

It is stable in liquid form below 50°C (Fig.4).

What are the optimal pH and temperature for FADGDH-AA?

The optimal pH is 7.0–7.5, and the optimal temperature is 40–50°C.

What is the molecular weight of FADGDH-AA?

It exhibits a monomeric structure with a molecular weight of approximately 90 kDa (SDS-PAGE).

What are the main applications of FADGDH-AA?

It is useful for the determination of blood glucose levels in diabetes diagnosis, with minimal interference from substances such as maltose and xylose.

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