The Histamine test uses a rapid enzymatic assay that is AOAC-validated, easy-to-use and provides a quantitative test result in less than 1 hr. Histamine Test is validated assay for the determination of histamine in tuna, bonito, canned tuna, fish sauce and other products.
Feature
- Simple Just dissolve the reagents and mix with the sample. The analysis is simple and safe. Due to high specificity of the enzyme, no hazardous chemicals-such as strong acids or organic solvents-are needed.
- Rapid The reaction takes only 15 minutes. For raw fish samples, the test can be fully completed-from sample preparation to result- in one hour.
- Accurate AOAC Performance Tested Method(PTM)Certification. The test has been shown to have a high correlation with official laboratory reference methods.
→Certification PDF file from here
Histamine Test (AOAC-RI PTM) - Instruction Video -
Casestudy
Case1: Fishery Processing Plant
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Histamine can be measured easily and quickly with high accuracy by simple pretreatment of specimen and pipetting.
Background and Implementation:
A major tuna processing company in Japan, implemented "Histamine Test" to enhance their quality control processes.
The decision was driven by:
- Need for cost reduction in voluntary inspections
- Desire to shorten the time required to obtain test results
- Alignment with the Maruha Nichiro Group's "Guidelines for Histamine Management"
Effects and Benefits:
- Significant Time Savings:
- Reduced testing time from up to two weeks to just a few hours - Cost Efficiency:
- Enabled in-house testing, reducing expenses associated with outsourced inspections - Improved Quality Control:
- Allows for testing of about 10 raw material samples per week - Compliance with Standards:
- Helps meet benchmark values based on FDA and Codex Alimentarius Commission standards (50 ppm for raw materials, 200 ppm for finished products) - Ease of Use:
- Simple operation allows accurate testing without specialized knowledge or skills - Scalability:
- Simplicity of the test kit enables training of more staff to handle increasing sample numbers - International Credibility:
- AOAC-RI PTM certification provides high confidence in test results for international distribution
Case2: Katsuobushi (dried bonito) Manufacturer
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(1)Homogenize the sample and measure to 1 g.
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(2)To 1 g of the sample, add 24 mL of sample treatment buffer, stir, heat with boiling water for 20 min, and then cool down.
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(3)Filter with paper flter and obtain a test solution.
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(4)To the test solution, add several reagents avoid light,and incubate at 37℃ for 15 min.
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(5)Measure the absorbance of the test solution at 470nm. Calculate the level of histamine using a provided formula.
Background and Implementation:
A renowned manufacturer of Katsuobushi (dried bonito) in Japan, implemented "Histamine Test" to enhance their quality control processes.
The decision was driven by:
- Need for quick and cost-effective in-house histamine testing
- Desire to perform tests at various stages of production
- Requirement for efficient testing of multiple samples
Effects and Benefits:
- Rapid Results:
- Enables quick decision-making for product shipment and production restart - Cost Efficiency:
- Reduced expenses associated with outsourced testing
- Decreased warehousing time and costs due to faster result turnaround - Comprehensive Quality Control:
- Allows testing at multiple stages, from raw materials to final products
- Facilitates periodic and unannounced checks for consistent quality assurance - Increased Testing Capacity:
- Enables testing of 15-20 samples daily with ease
- Allows for extensive sampling (up to 100 samples) to verify cooling/freezing effectiveness - Data Accumulation:
- Facilitates the collection of abundant data, useful for improving hygiene control - Reliability:
- Comparative tests showed accuracy equivalent to the AOAC method - HACCP Compliance:
- Supports HACCP-based operations by providing quick, reliable histamine data - Versatility:
- Simple enough for one examiner to perform tests from early morning, with reports ready by noon
Recommended Equipment
When you use the "Histamine Test", we recommend the use of the following equipment. Also available in a general spectrophotometer.
Absorptiometer RGB (Model DPM2-ABS)
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Product Specification
| Product Name | Histamine Test |
|---|---|
| Product Code | 61341 |
| Number of measurements | 60 assays |
| Composition |
Enzyme reagent (lyophilisate)x 6 |
| Storage | 2-8 ℃ |
| Expiry | End of month after 31 months from manufacturing date. |
Caution
- The reagent can not be used for purposes other than voluntary health inspection and research applications.
- This measurement, instruments and EDTA solution such as a micro pipette in addition to this kit and the absorbance meter is required.
- Histamine is easy to adsorb to the glass. Please use the plastic tube or the like.
- This kit is a histamine assay kit for raw fish. However, by varying the pretreatment method, it is the case that can be adapted to samples other than raw fish.
Award history
- Received the Technical Achievement Award in Fisheries Science from the Japanese Society of Fisheries Science.(2007)
- Received the Japan Soy Sauce Technology Award in application section from the Japan Soy Sauce Association.(2014)
- New! Histamine Test certificated the AOAC Performance Tested Method(PTM).
References
Validation study of histamine test for the determination of histamine in selected fish products. J. AOAC Int. 2019, 102, 164-180.
Simple and rapid determination of histamine in food using a new histamine dehydrogenase from Rhizobium sp. Anal. Biochem. 2005. 346, 320-326.