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ATP Depletion-The Risk of False-Negatives in Hygiene Monitoring
Professor and Chair of the Food Science Department University of Wisconsin - Madison Scott A. Rankin, Ph.D.
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Case Study: ATP Test (Kikkoman A3) in food and beverage industry
Director of Key Accounts and Market Segments, Weber Scientific Marybeth Karczynski
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Introduction of Kikkoman
General Manager, Marketing & Planning Division, Kikkoman Biochemifa Company Mikio Bakke, Ph.D.
Outline
| Product | ATP Test (Kikkoman A3): LuciPac A3 Surface |
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| Event date | 2022.08.18 |
Seminar Detail
| Venue | Webinar: Food Safety Magazine |
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Result contents
ATP tests for hygiene monitoring are used in food processing because they are fast, easy to use, and cost-effective. Recent research has characterized the limitations of conventional ATP tests—limitations that can lead to false negative hygiene assessments. The issue relates to the tendency of ATP to degrade in many soils and under common plant environmental conditions. If this depletion occurs, ATP-based assessments become increasingly less sensitive and may yield indications that the surface in question is clean when, in fact, significant levels of soil may still remain. Conditions resulting in ATP depletion are most common in typical manufacturing venues and can result in a false negative in areas sensitive to food hygiene maintenance. This webinar will present recent research from the University of Wisconsin–Madison demonstrating this ATP-depletion phenomenon and how processors can guard against such false negative assessments.
Learning objectives:
- Why ATP tests may show false negative assessments
- Which food, and which food processors, are most at risk for these false negative assessments
- How this issue with ATP may be impacting your sanitation program
Related seminars
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Event Overview
HyServe will be hosting an upcoming webinar as part of the A3 Webinar Series.
Dates and Times (CET):
- English Session: Jan 27, 2026 | 11:00-12:00
- Spanish Session: January 29, 2026 | 16:30 – 18:00
- Portuguese Session: January 30, 2026 | 14:00 – 15:00
Are you confident your HACCP plan can move from a binder on a shelf to verifiable, audit-ready proof in seconds?
Managing food safety with manual logs and subjective visual checks is time-consuming, prone to errors, and difficult to defend during an audit. When a deviation occurs, how quickly can you "close the loop" to prove your corrective action was effective? Join our upcoming webinar to learn how to automate and strengthen your entire food safety program. We will explore how Kikkoman's A3 technology and the Lumitester App directly support the most critical "action" principles of HACCP—Monitoring, Corrective Actions, and Verification.
What You Will Learn
In this webinar, we will cover:
- Why Superior Sensitivity Matters: How Kikkoman's A3 technology (measuring ATP+ADP+AMP) finds contamination from processed or heated foods that standard ATP-only tests miss.
- Automate Monitoring (Principle 5): Move from subjective "visual checks" to objective, numerical RLU data for a consistent standard of "clean".
- "Close the Loop" on Corrective Actions (Principle 6): Use real-time results to trigger an immediate corrective action (re-clean) and instantly re-test to verify it was successful.
- Achieve Total Accountability (Principle 7): Eliminate "messy, error-prone, and falsifiable" paper logs. See how every test is automatically saved to the cloud with the operator ID, time, date, and location, creating an unchangeable, audit-ready digital record.
- Verify Your System: Use the app's data to instantly identify sanitation trends, validate cleaning procedures, and prove your HACCP plan is working effectively.
Target Audience
- Quality Assurance Managers
- HACCP Coordinators
- Food Safety Professionals
looking to save time, reduce risk, and create a truly defensible food safety system.
Speaker Information
- Jorge E. Padilla
- Stella Maciel
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- Event date
- 2025.07.31
- Product
- ATP Test (Kikkoman A3): LuciPac A3 Surface, LuciPac A3 Water, LuciPac A3 Pre-moisten
- Venue
- Microsoft Teams
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- Event date
- 2025.05.28
- Product
- ATP Test (Kikkoman A3): LuciPac A3 Surface, LuciPac A3 Water, LuciPac A3 Pre-moisten
- Venue
- Facebook Live