The enzyme is useful for the determination of d-glucose in clinical analysis and self-monitoring blood glucose meters.
| 系统名称 | D-Glucose : acceptor 1-oxidoreductase |
|---|---|
| EC编号 | 1.1.5.9 |
| 反应式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
| Appearance | Yellow lyophilizate | |
|---|---|---|
| Activity | ≧700 U/mg lyophilizate | |
| Contaminant | NAD Glucose Dehydrogenase ≺1.0×10-2％ | |
| Hexokinase ≺1.0×10-2％ | ||
| α-Glucosidase ≺1.0×10-2％ | ||
| β-Glucosidase ≺1.0×10-2％ | ||
| Storage | below -20℃ protected from light |
| Molecular weight | ca. 85 kDa (SDS-PAGE) |
|---|---|
| Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
| Michaelis constant | 2.2×10-2M (D-Glucose) |
| pH Optimum | 7.0–7.5 |
| pH Stability | 4.5–8.0 |
| Optimum temperature | 40–50℃ |
| Thermal stability | below 45℃ |
| Specificity | D-glucose (100), Maltose (≺0.3), |
| D-xylose (≺10), D-galactose (≺1.2) | |
| sucrose (≺0.0) |
The enzyme is useful for the determination of D-glucose in clinical analysis and self-monitoring blood glucose meters.
Hatada M, Loew N, Inose-Takahashi Y, Okuda-Shimazaki J, Tsugawa W, Mulchandani A, Sode K (2018)
Development of a glucose sensor employing quick and easy modification method with mediator for altering electron acceptor preference.
Bioelectrochemistry, 121, 185-190
Okuda-Shimazaki, J, Yoshida H, Sode K (2020)
FAD dependent glucose dehydrogenases – Discovery and engineering of representative glucose sensing enzymes –
Bioelectrochemistry, 132, 107414
