臨床検査および持続血糖測定(CGM)において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
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系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
臨床検査および持続血糖測定(CGM)において、D-グルコースの測定に利用されます。
由来 | recombinant A. sojae |
---|---|
系統名 | D-Glucose : acceptor 1-oxidoreductase |
EC 番号 | 1.1.5.9 |
反応式 | D-Glucose + acceptor →→→ D-Glucono-1,5-lactone + reduced acceptor |
Appearance | yellow to brown lyophilizate |
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Activity | ≧700 U/mg |
Contaminants | NAD glucose dehydrogenase <0.01 U/U% |
Hexokinase <0.01 U/U% | |
α-glucosidase <0.01 U/U% | |
β-glucosidase <0.01 U/U% | |
Storage condition | below -20℃ protected from light |
Molecular weight | ca. 90 kDa (SDS-PAGE) |
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Structure | monomer, one mole of FAD per mole of enzyme glycoprotein |
Michaelis constant | 6.4×10-2M (D-glucose) |
pH Optimum | 7.0–7.5 |
pH Stability | 2.5–9.5 |
Optimum temperature | 45℃ |
Thermal stability (liquid form) | below 60℃ |
Thermal stability (powder form) | stable at 30℃ for at least one month |
Inhibitors | Mn2+, Ag+ |
Specificity | D-glucose (100%), maltose (0.2%), D-xylose (0.9%), D-galactose (0.8%) sucrose (<0.1%), D-mannose (0.4%) 2-deoxy-D-glucose (23.5%) |
FADGDH-AD is useful for the determination of D-glucose in clinical analysis and continuous glucose monitoring (CGM) meter for diabetes patients. The FADGDH-AD was developed as an oxygen-insensitive alternative to Glucose oxidase (GOD) with its high stability for the applications such as bioelectrodes in glucose sensing device and glucose enzymatic fuel cells.
Satake R, Ichiyanagi A, Ichikawa K, Hirokawa K, Araki Y, Yoshimura T, Gomi K (2015)
Novel glucose dehydrogenase from Mucor prainii: Purification, characterization, molecular cloning and gene expression in Aspergillus sojae
Biosci Bioeng., 120, 498-503
Masakari Y, Hara C, Araki Y, Gomi K, Ito K (2020)
Improvement in the thermal stability of Mucor prainii-derived FAD-dependent glucose dehydrogenase via protein chimerization
Enzyme Microb Technol., 132, 109387, doi: 10.1016/j.enzmictec.2019.109387.
https://doi.org/10.1016/j.enzmictec.2019.109387, (cited 2020-07-02)