Glucose Dehydrogenase (FADGDH-AB)

常见问题解答

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

临床检测用酶

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FADGDH-AB be stored?

Please store at or below -20°C.

How is the activity of FADGDH-AB measured?

An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.

What is the substrate specificity of FADGDH-AB?

It shows less than 1% relative activity toward maltose compared to 100% for glucose.

What stabilizers are used in FADGDH-AB?

No stabilizers are used.

How thermostable is FADGDH-AB?

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at 30°C for at least 20 days (Fig.5).

What are the optimal pH and temperature for FADGDH-AB?

The optimal pH is 7.0–7.5, and the optimal temperature is 40–50°C.

What is the molecular weight of FADGDH-AB?

It exhibits a monomeric structure with a molecular weight of approximately 85 kDa (SDS-PAGE).

What are the main applications of FADGDH-AB?

It is useful for the determination of blood glucose levels in diabetes diagnosis, with minimal interference from substances such as maltose.

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