Glucose Dehydrogenase (FADGDH-AB)
常见问题解答
- Where can I get Safety Data Sheet (SDS) of products?
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SDS and specification documents are available on each product page. They are also available from HERE. ⇒
临床检测用酶
- What precautions should be taken when handling the product after opening?
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To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)
- How should FADGDH-AB be stored?
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Please store at or below -20°C.
- How is the activity of FADGDH-AB measured?
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An example of activity measurement: The dehydrogenase activity is assayed in a reaction mixture containing glucose as a substrate, phenazine methosulfate as a mediator and dichlorophenolindophenol (DCIP). The activity is calculated by monitoring the decrease in the absorbance of DCIP at 600 nm.
- What is the substrate specificity of FADGDH-AB?
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It shows less than 1% relative activity toward maltose compared to 100% for glucose.
- What stabilizers are used in FADGDH-AB?
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No stabilizers are used.
- How thermostable is FADGDH-AB?
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It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at 30°C for at least 20 days (Fig.5).
- What are the optimal pH and temperature for FADGDH-AB?
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The optimal pH is 7.0–7.5, and the optimal temperature is 40–50°C.
- What is the molecular weight of FADGDH-AB?
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It exhibits a monomeric structure with a molecular weight of approximately 85 kDa (SDS-PAGE).
- What are the main applications of FADGDH-AB?
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It is useful for the determination of blood glucose levels in diabetes diagnosis, with minimal interference from substances such as maltose.