Fructosyl-peptide Oxidase (FPOX-CET)

常见问题解答

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

临床检测用酶

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C,

How should FPOX-CET be stored?

Please store at or below -20°C.

How is the activity of FPOX-CET measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl glycine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The production of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FPOX-CET?

It shows 100% activity toward fructosyl valyl histidine (F-ValHis), 150% toward Fructosyl-glycine (F-Gly) and 68.6% toward ε-fructosyl lysine (εF-Lys).

What protease is used in combination with FPOX-CET for HbA1c measurement?

Please contact sales representative.

What stabilizers are used in FPOX-CET?

Sodium glutamate and EDTA are used as stabilizers.

How thermostable is FPOX-CET?

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at -20°C for at least twelve months (Fig.5).

What are the optimal pH and temperature for FPOX-CET?

The optimal pH is 7.5–8.5, and the optimal temperature is 37–45°C.

What is the molecular weight of FPOX-CET?

The molecular weight is approximately 60 kDa (gel filtration), and it exhibits a monomeric structure of 52 kDa (SDS-PAGE).

What are the main applications of FPOX-CET?

It is useful for the determination of HbA1c, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

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