SDS and specification documents are available on each product page. They are also available from HERE. ⇒

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

Please store at or below -20°C.

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl glycine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The production of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

It shows 100% activity toward fructosyl valyl histidine (F-ValHis), 84% toward ε-fructosyl lysine (εF-Lys) and 53% toward Fructosyl-glycine (F-Gly).

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Sodium glutamate and EDTA are used as stabilizers.

It is stable in liquid form below 45°C (Fig.4). In powder form, it remains stable at 37°C for at least one month (Fig.5).

The optimal pH is 7.5–8.0, and the optimal temperature is 35–42°C.

The molecular weight is approximately 60 kDa (gel filtration), and it exhibits a monomeric structure of 52 kDa (SDS-PAGE).

It is useful for the determination of HbA1c, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.