Fructosyl-amino Acid Oxidase (FAOD-E)

常见问题解答

Where can I get Safety Data Sheet (SDS) of products?

SDS and specification documents are available on each product page. They are also available from HERE. ⇒

临床检测用酶

What precautions should be taken when handling the product after opening?

To avoid moisture absorption of the enzyme powder, please allow it to return to room temperature in a sealed state before use. (Recommended conditions: around 25°C, humidity below 55%)

How should FAOD-E be stored?

Please store at or below -20°C.

How is the activity of FAOD-E measured?

An example of activity measurement: The oxidase activity is assayed in a reaction mixture containing fructosyl valine as a substrate, peroxidase, 4-aminoantipyrine and TOOS. The prouction of hydrogen peroxide is colorimetrically evaluated by measuring absorbance at 555 nm.

What is the substrate specificity of FAOD-E?

It shows 100% activity toward ε-fructosyl lysine (εF-Lys), 65% toward fructosyl valine (F-Val), and 30% toward fructosyl glycine (F-Gly).

What stabilizers are used in FAOD-E?

Trehalose is used as a stabilizer.

How thermostable is FAOD-E?

It is stable in liquid form below 30°C (Fig.4) and remains stable at 25°C for at least two weeks (Fig.5). In powder form, it remains stable at 37°C for at least three weeks (Fig.6).

What are the optimal pH and temperature for FAOD-E?

The optimal pH is 8.0–8.5, and the optimal temperature is 35–40°C.

What is the molecular weight of FAOD-E?

The molecular weight is approximately 45 kDa (gel filtration), and it exhibits a monomeric structure of 50 kDa (SDS-PAGE).

What are the main applications of FAOD-E?

It is useful for the determination of glycated albumin, an indicator for diabetes diagnosis and blood glucose control, in clinical analysis.

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